1.Synthesize both phospho and non-phospho peptides.
Services
Time (cost)
IV. Additional Bleed-Out Service
provide 50 ml from each rabbit
1 week (+$150/per rabbit)
V. Re-boosting Immunizations (two additional booster injection, 20 days interval, 50 ml antiserum bleed after 50 days)
7 weeks
(+$250/per rabbit)
VI. ELISA Test
ELISA test for final total bleed (including conjugating peptide to BSA)
1 week
(+$100)
VII. Purification
Synthesis the control peptide of the same sequence without phospho residue.
Phospho-peptide antibody purification by 2-steps peptide affinity purification including phospho-peptide column and non-phospho-peptide column
ELISA data will be included for free
1 week
(+$1200)
Phase
Time (cost)
I. Peptide synthesis
Peptide synthesis up to 15 amino acids.
Each phospho-residue (Phospho-Ser, Tyr or Thr)
2 week
(additional $150/per site)
II. Conjugation
Conjugation of 5 mg peptide (using Cysteine) to KLH.
10 mg free peptide will be provided for client
1 week
III. Immunization and anti-sera production
Ten-week protocol to produce antibodies in 2 rabbits.
Pre-immune 5 ml/per rabbit and total final bleeds from two rabbits (100 ml antiserum) will be provided to client.
10 weeks
Total
13 weeks ($950)
Additional Options
Phosphospecific Antibody Affinity Purification
Antagene Inc. offers phosphospecific antibody affinity purification including:
1. Phosphospecific peptide synthesis (up to 15 amino acids)--10 mg
used for phosphospecific epitope affinity purification, 10 mg used
for antibody production, and 10 mg delivered to client
2. 10 mg non-phosphospecific peptide (up to 15 amino acids) used
for affinity depletion
3. Immunization of 2 rabbits
4. Phosphospecific and non-phosphospecific peptide ELISA titration
of antibody for final bleeds
5. Pre-immune sample of serum (5 mL/each rabbit) and anti-serum
(100mL total) from two rabbits
6. Affinity purification of serum Shipment of purified phosphospecific
antibody which does not cross-react with non-phosphospecific peptides
(confirmed by ELISA)
Notes
Antagene Inc will provide the customer with the service in the shortest possible time. Delays may be incurred if the antigen is poorly immunogenic .
All the products of this project shall become the exclusive property of the client at the conclusion of the project. Full confidentiality is guaranteed.
Please contact us for a quotation of your customized phospho-antibody development project or