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Phospho-Antibody Production- $2,120/ project

1.Synthesize both phospho and non-phospho peptides.

Services	Time (cost)
IV. Additional Bleed-Out Service provide 50 ml from each rabbit	1 week (+$150/per rabbit)
V. Re-boosting Immunizations (two additional booster injection, 20 days interval, 50 ml antiserum bleed after 50 days)	7 weeks (+$250/per rabbit)
VI. ELISA Test ELISA test for final total bleed (including conjugating peptide to BSA)	1 week (+$100)
VII. Purification Synthesis the control peptide of the same sequence without phospho residue. Phospho-peptide antibody purification by 2-steps peptide affinity purification including phospho-peptide column and non-phospho-peptide column ELISA data will be included for free	1 week (+$1200)

Phase	Time (cost)
I. Peptide synthesis Peptide synthesis up to 15 amino acids. Each phospho-residue (Phospho-Ser, Tyr or Thr)	2 week (additional $150/per site)
II. Conjugation Conjugation of 5 mg peptide (using Cysteine) to KLH. 10 mg free peptide will be provided for client	1 week
III. Immunization and anti-sera production Ten-week protocol to produce antibodies in 2 rabbits. Pre-immune 5 ml/per rabbit and total final bleeds from two rabbits (100 ml antiserum) will be provided to client.	10 weeks
Total	13 weeks ($950)

Additional Options

Phosphospecific Antibody Affinity Purification

Antagene Inc. offers phosphospecific antibody affinity purification including:

1. Phosphospecific peptide synthesis (up to 15 amino acids)--10 mg
    used for phosphospecific epitope affinity purification, 10 mg used
    for antibody production, and 10 mg delivered to client

2. 10 mg non-phosphospecific peptide (up to 15 amino acids) used
    for affinity depletion

3. Immunization of 2 rabbits

4. Phosphospecific and non-phosphospecific peptide ELISA titration
     of antibody for final bleeds

5. Pre-immune sample of serum (5 mL/each rabbit) and anti-serum
     (100mL total) from two rabbits

6. Affinity purification of serum Shipment of purified phosphospecific
    antibody which does not cross-react with non-phosphospecific peptides
    (confirmed by ELISA)

Notes
Antagene Inc will provide the customer with the service in the shortest possible time. Delays may be incurred if the antigen is poorly immunogenic .

All the products of this project shall become the exclusive property of the client at the conclusion of the project. Full confidentiality is guaranteed.

Please contact us for a quotation of your customized phospho-antibody development project or

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2. Conjugate of phosphorylated peptide.

3. Immunize animals.

4. Collect antisera.

5. Bind phosphorylated and non-phosphorylated to affinity chromatography columns.

6. Titer antibodies against phosphorylated and non-phosphorylated peptides.

Custom Phospho-Specific Polyclonal Antibody complete service