| Phase | Time (Cost) |
The client may choose to follow our standard immunization protocol or to use your own protocol. In most cases, we injected the mouse every two weeks for a total of 6 weeks before doing a test bleed on the mouse. The test bleed samples are then examined for antibody production using ELISA (The client may request up to 0.5 ml serum sample/per mouse for their own additional characterization). ELISA will be used to determine the highest titer mice to be used for cell fusion. The immunization process may be extended if the test result is not optimal. |
8-10 weeks ($1,000 without peptide synthesis) or ($1,500 with peptide synthesis) |
Phase 2: Fusion Fusion of spleen cells with myeloma cell line. The fused cells are then placed in 96-well culture plates. Viable colonies appear as early as 3-5 days after fusion and are kept healthy with a fresh supply of nutrient media as necessary. |
2-6 weeks $1,000 |
Phase 3: Screening When hybridoma colonies are large enough to screen, screening of all wells containing fusion products is done with ELISA assay. All positive wells will be expanded and frozen for parental hybridomas. The number of positives depends on the nature of the antigen, and can range from a few hybridomas to a dozen or more. Additional hybrids can be frozen for further studies. |
2-6 weeks $1,000 |
Phase 4: Stabilization and Expension of clones After positive hybridomas are selected for further development, they will be cloned using the limited dilution method and screened by ELISA. 5 clones with the desired specificity will be expanded, and hybridoma medium (50 - 100 ml) will be collected for further studies |
2-4 weeks $1,000 |
Total |
17-29 weeks ($3,999) |
|
1 week $500/per project |
