Antibody Service List & Price
Polyclonal Antibody(Tab1),Monoclonal Antibody(Tab2),Phosphospecific Antibody(Tab3),Abs Service Price
Custom Antibody Polyclonal Production – $999or $699 (antigen provided by client)
| Phase | Time (cost) |
| I. Peptide Design and Synthesis We start with the synthesis of custom peptide for up to 15 amino acids. ($12 for each additional residue).Special Assistants are provided for peptide design free of charge. | 2 weeks |
| II. Conjugation It takes 1 week to conjugate 10 mg of peptide to KLH (using Cysteine). We will provide 10 mg of peptide to the client for free. | 1 week |
| III. Immunization & Anti-Sera Production The Conjugated Peptides ( or recombinant proteins) are then used to immunize rabbits. It takes a total of 10 weeks to develop the antibodies from 2 rabbits. The pre-immune serum (5ml/per rabbit) and the combined final bleeds from the two rabbits (100ml antiserum) will be delivered to the client. | 10 weeks |
Total | 13 weeks ($899) |
Custom Monoclonal Antibody – $3,999 (complete service)
| Phase | Time (Cost) |
Phase 1: Immunization1. Immunization of Balb/c mice (up to 5 animals) with antigen to develop antiserums. The client may choose to follow our standard immunization protocol or to use your own protocol. In most cases, we injected the mouse every two weeks for a total of 6 weeks before doing a test bleed on the mouse. The test bleed samples are then examined for antibody production using ELISA (The client may request up to 0.5 ml serum sample/per mouse for their own additional characterization). ELISA will be used to determine the highest titer mice to be used for cell fusion. The immunization process may be extended if the test result is not optimal. Antigen requirement: 0.5 mg of purified recombinant protein or intact protein will be required for immunization. With peptide synthesis request: Custom Peptide synthesis up to 15 amino acids (additional amino acid for $12 per residue) with HPLC purification up to 90% purity, peptide KLH conjugation for immunization, peptide BSA conjugation for screen. 10 mg free peptide will be available for the client. | 8-10 weeks ($1,000 without peptide synthesis) or ($1,500 with peptide synthesis) |
Phase 2: Fusion Fusion of spleen cells with myeloma cell line. The fused cells are then placed in 96-well culture plates. Viable colonies appear as early as 3-5 days after fusion and are kept healthy with a fresh supply of nutrient media as necessary. | 2-6 weeks $1,000 |
Phase 3: Screening When hybridoma colonies are large enough to screen, screening of all wells containing fusion products is done with ELISA assay. All positive wells will be expanded and frozen for parental hybridomas. The number of positives depends on the nature of the antigen, and can range from a few hybridomas to a dozen or more. Additional hybrids can be frozen for further studies. | 2-6 weeks $1,000 |
| Phase 4: Stabilization and Expension of clones After positive hybridomas are selected for further development, they will be cloned using the limited dilution method and screened by ELISA. 5 clones with the desired specificity will be expanded, and hybridoma medium (50 – 100 ml) will be collected for further studies | 2-4 weeks $1,000 |
| Total | 17-29 weeks ($3,999) |
Additional Options
Purification The secreted antibody will be purified by Protein G affinity chromatography and deliver to the customer (Approximately 20-30 mg). Antibodies are eluted using both pH11 and pH2.5 buffers and neutralized immediately followed by dialysis in PBS | 1 week $500/per project |
Notes:
Antagene Inc will provide the customer with the service in the shortest possible time. Delays may be incurred if the antigen is poorly immunogenic .
All the products of this project shall become the exclusive property of the client at the conclusion of the project. Full confidentiality is guaranteed.
Please contact us for a quotation of your customized monoclonal antibody development project or go to shop
Phosphospecific-Antibody Production- $2,400/ project
1. Synthesize both phospho and non-phospho peptides.
2. Conjugate of phosphorylated peptide.
3. Immunize animals.
4. Collect antiserum.
5. Bind phosphorylated and non-phosphorylated to affinity chromatography columns.
6. Titer antibodies against phosphorylated and non-phosphorylated peptides
Custom Phospho-Specific Polyclonal Antibody complete service
| Phase | Time (cost) |
| I. Peptide synthesis Peptide synthesis up to 15 amino acids. Each phospho-residue (Phospho-Ser, Tyr or Thr) | 2 week (additional $150/per site) |
| II. Conjugation Conjugation of 5 mg peptide (using Cysteine) to KLH. 10 mg free peptide will be provided for client | 1 week |
| III. Immunization and anti-sera production Ten-week protocol to produce antibodies in 2 rabbits. Pre-immune 5 ml/per rabbit and total final bleeds from two rabbits (100 ml antiserum) will be provided to client. | 10 weeks |
Total | 13 weeks ($950) |
Additional Options
| Services | Time (cost) |
| IV. Additional Bleed-Out Service provide 50 ml from each rabbit | 1 week (+$150/per rabbit) |
| V. Re-boosting Immunizations (two additional booster injection, 20 days interval, 50 ml antiserum bleed after 50 days) | 7 weeks (+$250/per rabbit) |
| VI. ELISA Test ELISA test for final total bleed (including conjugating peptide to BSA) | 1 week (+$100) |
| VII. Purification Synthesis the control peptide of the same sequence without phospho residue. Phospho-peptide antibody purification by 2-steps peptide affinity purification including phospho-peptide column and non-phospho-peptide column ELISA data will be included for free | 1 week (+$1200) |
Phosphospecific Antibody Affinity Purification
Antagene Inc. offers phosphospecific antibody affinity purification including:
1. Phosphospecific peptide synthesis (up to 15 amino acids)–10 mg
used for phosphospecific epitope affinity purification, 10 mg used
for antibody production, and 10 mg delivered to client
2. 10 mg non-phosphospecific peptide (up to 15 amino acids) used
for affinity depletion
3. Immunization of 2 rabbits
4. Phosphospecific and non-phosphospecific peptide ELISA titration
of antibody for final bleeds
5. Pre-immune sample of serum (5 mL/each rabbit) and anti-serum
(100mL total) from two rabbits
6. Affinity purification of serum Shipment of purified phosphospecific
antibody which does not cross-react with non-phosphospecific peptides
(confirmed by ELISA)
Notes
Antagene Inc will provide the customer with the service in the shortest possible time. Delays may be incurred if the antigen is poorly immunogenic .
All the products of this project shall become the exclusive property of the client at the conclusion of the project. Full confidentiality is guaranteed.
Please contact us for a quotation of your customized phospho-antibody development project or go to shop
| Services | Time (cost) |
| Polyclonal antibody production, Complete service | $999/project |
| Phospho-peptide polyclonal antibody production and phospho-peptide affinity purification, Complete service | $2,400/project |
| Peptide synthesis | Starts from $5/AA |
| Phospho-peptide synthesis | $150/per site |
| Peptide conjugation to KLH | $160/peptide |
| Immunization and antisera production without peptide synthesis | $699/antigen |
| Titer test by ELISA | $100/per project |
| Western blotting (WB) test | $200/per gel |
| Antibody purification by protein G | $500/per project |
| Antibody purification by peptide affinity column | $600/per project |
| Phospho-peptide antibody purification by 2-steps peptide affinity purification including phospho-peptide column and non-phospho-peptide column | $1,200/per project |
| Polyclonal antibody production: additional rabbit | $250/rabbit |
| Polyclonal antibody production: extended protocol | $350/rabbit |
| Monoclonal antibody production, Complete service | $3,999/project |
| Phospho-specific monoclonal antibody production, Complete service | $6,000/project |
| Monoclonal antibody production: peptide conjugation to KLH | $160/peptide |
| Monoclonal antibody production: immunization and antisera production | $1,500/antigen |
| Monoclonal antibody production: hybridoma fusion and screening of clones | $2,000/fusion |
| Monoclonal antibody production: screening of phospho-specific clones | $3,000/fusion |
| Monoclonal antibody production: Stabilization of clones and expansion of clones. Relevant clones(single cells) are frozen at this stage and hybridoma medium(500-100ml) collected for further studies | $1500/per project |
| Monoclonal antibody production: Isotyping of clones and ascites production. Isotypes of all selected clones will be determined and production of ascites from 5 mices if needed | $1500/per project |
| Monoclonal antibody production: large scale culture up to 200 ml | $500/clone |
| Monoclonal antibody service: ascites production up to 20 ml | $500/clone |
| Monoclonal antibody service: antibody purification by protein G | $500/clone |