Antibody Polyclonal Production | Custom Antibody Production

Phase	Time (cost)
I. Peptide Design and Synthesis We start with the synthesis of custom peptide for up to 15 amino acids. ($12 for each additional residue).Special Assistants are provided for peptide design free of charge.	2 weeks
II. Conjugation � It takes 1 week to conjugate 10 mg of peptide to KLH (using Cysteine). � We will provide 10 mg of peptide to the client for free.	1 week
III. Immunization & Anti-Sera Production ·The Conjugated Peptides ( or recombinant proteins) are then used to immunize rabbits. · It takes a total of 10 weeks to develop the antibodies from 2 rabbits. · The pre-immune serum (5ml/per rabbit) and the combined final bleeds from the two rabbits (100ml antiserum) will be delivered to the client.	10 weeks
Total	13 weeks ($899)

Services	Time (cost)
IV. Additional Bleed Out � Bleed out 50 ml from each rabbit	1 week (+$250/per rabbit)
V. Re-boosting (two additional booster injection on a 20 days interval) � 50 ml antiserum (bleed after 50 days)	7 weeks (+$350/per rabbit)
VI. ELISA Test � Conjugation of peptide to BSA � ELISA test for final total bleed	1 week (+$100)
VII. Purification � Peptide-specific affinity purification � ELISA data included.	1 week (+$600)

*Notes
Antagene Inc will provide the customer with the service in the shortest possible time. Delays may be incurred if the antigen is poorly immunogenic .

All the products of this project shall become the exclusive property of the client at the conclusion of the project. Full confidentiality is guaranteed.

Please contact us for a quotation of your customized polyclonal antibody development project

Order Online

Subject:	New Zealand White Rabbits
Adjuvant:	Started with the Complete Freund's Adjuvant (known as CFA), followed by the Incomplete Freund's Adjuvant (known as IFA) for all subsequent injections.
Immunogen:	Immunogen are typically proteins and conjugated peptides. We used 0.5 mg of immunogen/rabbit/injection for each immunization.
Procedure:	The immunogen is diluted to 1 mL with sterile saline and combined with 1 mL of the appropriate adjuvant. The antigen and adjuvant are mixed to form a stable emulsion which is injected subcutaneously and provides enhanced immune response from the sustained presence of the immunogen.
Bleeds:	Blood is collected from the central ear artery with a 19 gauge needle and allowed to clot and retract at 37�C overnight. The clotted blood is then refrigerated for 24 hours before the serum is decanted and clarified by centrifugation at 2500 rpm for 20 minutes.
Schedule:	Day 1: Bleed 25 mL (yields 10 mL pre-immune serum). 1^st Immunize with antigen in CFA. Day 20: 2^nd Immunize with antigen in IFA. Day 40: 3^rd Immunize with antigen in IFA. Day 50: Test Bleed 10 mL for ELISA screen(internal quality control only, not provide to client). Day 60: 4^th Immunize with antigen in IFA. Day 70: Final Total Bleed 50 mL from Each Rabbit.