Ni-Pentadentate Agarose Kit for Purification of 6x His-tagged Proteins,Cat. #: REA005

Features:
-One-step purification from crude lysate to >99% pure protein
-High binding affinity, high capacity, and high specificity
-Purification under native or denaturing conditions
-No leaching of Ni ions
-Ready-to-use for any scale of purification
Kit components:
Ni-pentadentate Agarose resin, lysis buffer, wash buffer, binding buffer, and elution buffer
Binding capacity: 20-30 mg/ml (300–500 nmol @ ~60 kDa)
Support: Sepharose CL-6B
Bead structure: Cross-linked, 6% agarose
Bead size: 50–160 μm
Form: 50% suspension in 25% ethanol, precharged with Ni2+
Principle:
The Ni-pentadentate Protein Purification System is based on the remarkable selectivity of patent pending
resin for proteins containing an affinity tag of six consecutive histidine residues — the 6xHis tag. This technol-
ogy allows one-step purification of almost any His-tagged protein from any expression system under native
or denaturing conditions. Ni-pentadentate, which has five chelation sites for nickel ions, binds nickel more
tightly than metal-chelating purification systems that only have three sites available for interaction with metal
ions. The extra chelation site prevents nickel-ion leaching and results in a greater binding capacity and protein
preparations with higher purity than those obtained using other metal-chelating purification systems. The Ni-
pentadentate system can be used to purify His-tagged proteins from any expression system including baculo-
virus, mammalian cells, yeast, and bacteria.

Product: His-tagged Sumo Protease

Product description or back ground:
Recombinant Yeast Ulp1 is a highlyactive
protease which specifically recognizes the
tertiary structure of ubiquitin-like protein,
SUMO rather than a short sequence as other
commonly used proteases such as Thrombin,
TEV, and EK. The protease can be used to
cleave the SUMO from recombinant fusion
proteins at 30oC or 4oC at pH 7.0-7.9. After
digestion, the His-Tagged Ulp1 can be easily
removed by His tag affinity chromatography.

Mag Protein A/G Nanoparticles, Price:$210

Quantity: 1ml, Concentration: 4mg/ml
Product Description
Mag Protein A/G nanoparticles are ideal for antibody purification
and immunoprecipitation assays. MagProtein A/G nanoparticles
recognize and efficiently bind to the Fc part of
immunoglobulin (Ig) following a short incubation. The generated
nanoparticle-antibody complex can be separated from the rest of the
sample by magnet. The retained antibody can be eluted from the
nanoparticles using an elution buffer.

Tini Spin Columns for D/A (elution volume: 5-30 micro L)

Application: Plasmid preparation from single colony; genomic DNA isolation cultured cells (101-105 cells), tissues, or LCM
samples; DNA for genotyping from mouse tail snip (1 mm) or ear puncture; DNA extraction from gel pieces; DNA clean-up or
concentration for samples of low concentration such as ChIP DNA and DNA of post enzymatic reactions; primer removal after
PCR.

LC-MS MASS SPECTROMETRY SERVICE

Our mass spectrometry service will analyze:

Small Molecules: – all types of small molecules using positive or negative ESI
Peptides and Proteins, up to 50kD (with protein deconvolution)
Nucleic acids
Our services include:
Full scan ESI or APCI MS ESI for simple MW determination $49
Tandem ESI or APCI MS/MS (structural fragmentation / confirmation) $59
Full scan LC-MS (LC separation with MS) $79
Tandem LC-MS/MS (LC separation with fragmentation / confirmation) $99
Charge state determination (ZoomScan) $49
Multi-dimensional LC-MS: LC-MS/MS/MS or LC-MSn Call

HPLC SERVICE (U V / Photo Diode Array HPLC)
Reverse-phase Photo Diode Array HPLC C18 HPLC Chromatogram $49
Fluorescence C18 RP-HPLC Detection $59

Optical Rotation (Specific optical rotations calculated / confirmed) $49

Speedy next day turnaround on all samples – samples sent back as PDF files
Confidential Technical support is available on all analyses
Less than 1 mg of sample required – send samples with our sample submission form
Discount for 10 samples or more ran at the same time